EK1A42

Human Aβ1-42 ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

说明书

在售SKU:70-EK1A42-96, 70-EK1A42-48, EK1A42

种属：人
样本类型：血清、血浆、细胞培养上清
检测样本体积：血清、血浆样本50μL,细胞培养上清100μL
灵敏度：13.42 pg/mL
检测范围：15.63-1000 pg/mL
回收率：70%-130%

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Size
库存号

ELISA试剂盒详细信息

商品名	Human Aβ1-42 ELISA Kit检测试剂盒（酶联免疫吸附法）
种属	人
检测方法	双抗体夹心法
检测样本类型	血清、血浆、细胞培养上清
检测样本体积	血清、血浆样本50μL,细胞培养上清100μL
灵敏度	13.42 pg/mL
线性范围	15.63-1000 pg/mL
精密度	板内变异系数：8.6%-11.9%;板间变异系数：9.4%-11.3%
回收率	70%-130%
平均回收率	99%
板式	96孔板，可拆
保存条件	2-8℃保存。已拆开：标准品-20℃保存，其它4℃。
运输条件	2-8℃冰袋运输
检测原理	本试剂盒采用双抗体夹心酶联免疫吸附（ELISA）检测技术。该方法的原理是：将特异性抗人Aβ1-40抗体预先包被在高亲和力的酶标板上。检测时，向酶标板孔中加入标准品、待测样本以及生物素标记的检测抗体，经孵育后，样本中的Aβ1-40会与固相抗体和检测抗体结合形成复合物。通过洗涤步骤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素（Streptavidin-HRP）。再次洗涤后，加入显色底物TMB，并避光孵育进行显色反应。此时，孔内颜色的深浅与样本中Aβ1-40的浓度呈正比。最后加入终止液终止反应，并在450 nm波长（参考波长为570–630 nm）下测定各孔的吸光度值。