EK1113

Human FGF-19 ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

  • 分子靶点:FGF19
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:50 μL
  • 灵敏度:1.56 pg/mL
  • 检测范围:15.63 pg/mL - 1000 pg/mL
  • 回收率:85% - 114%

在售SKU:70-EK1113-48, 70-EK1113-96

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描述

商品名

Human FGF-19 ELISA Kit (人成纤维细胞生长因子19 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:2.8% - 4.6%;板间变异系数:2.5% - 4.8%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

50 μL

灵敏度

1.56 pg/mL

检测范围

15.63 pg/mL - 1000 pg/mL

回收率

85% - 114%

平均回收率

0.99

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗FGF-19单克隆抗体的96孔聚苯乙烯酶标板
  • 人FGF-19冻干标准品
  • FGF-19检测抗体
  • 标准品稀释液
  • 辣根过氧化物酶标记的链霉亲和素
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人 FGF-19 抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的 FGF-19 与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物 TMB,避光显色。颜色反应的深浅与样本中 FGF-19 的浓度成正比。加入终止液终止反应,在 450 nm 波长(参考波长 570 - 630 nm)测定吸光度值。

Human FGF-19 ELISA Kit (人成纤维细胞生长因子19 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 FGF19 靶点信息

FGF19 分子靶点信息概述

  • 分子名:FGF19, fibroblast growth factor 19
  • 基因家族:Fibroblast growth factor family

FGF19 分子靶点综述

成纤维细胞生长因子19(FGF-19)在人类中由FGF-19基因编码。FGF-19调控胆汁酸的合成,在肝脏中通过FGFR4/Klotho-β受体复合物对CYP7A1产生抑制作用。在小鼠模型中发现,FGF-19也具有代谢作用,可影响葡萄糖和脂类代谢。因胆汁酸吸收障碍的慢性腹泻患者的FGF-19减少。在胆汁郁积患者的肝脏中也存在FGF-19。代谢综合征、非酒精性脂肪肝和胰岛素耐受患者的FGF-19表达减少。而经过Roux-en-Y胃旁路减肥手术的肥胖患者,其FGF-19上升至正常水平。

人 Human FGF19 分子靶点信息

人 Human FGF19靶点分子功能(预测)

Enables fibroblast growth factor receptor binding activity. Involved in several processes, including negative regulation of bile acid biosynthetic process; positive regulation of MAPK cascade; and positive regulation of glucose import. Acts upstream of or within fibroblast growth factor receptor signaling pathway and positive regulation of cell population proliferation. Predicted to be located in extracellular region. Predicted to be active in cytoplasm and extracellular space.

操作步骤


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引用文献


  1. Unconjugated and secondary bile acid profiles in response to higher-fat, lower-carbohydrate diet and associated with related gut microbiota: A 6-month randomized controlled-feeding trial 

  2. Clinical value of fibroblast growth factor 19 in predicting gastrointestinal dysfunction in patients with sepsis 

  3. Dietary fiber alleviates alcoholic liver injury via Bacteroides acidifaciens and subsequent ammonia detoxification 

  4. Unconjugated and Secondary Bile Acid Profiles in Response to Higher-Fat, Lower-Carbohydrate Diet and Associated with Related Gut Microbiota: A 6-Month Randomized Controlled-Feeding Trial 

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