Rat IL-6 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥10,800.00
- 分子靶点:IL6, IL-6, IFNB2, HGF
- 种属:大鼠 (Rat)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:20 μL
- 灵敏度:2.57 pg/mL
- 检测范围:31.25 pg/mL - 2000 pg/mL
- 回收率:92% - 104%
在售SKU:70-EK306/3-48, 70-EK306/3-96
描述
商品名 |
Rat IL-6 ELISA Kit (大鼠白细胞介素6 ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:4.2% - 7.7%;板间变异系数:6.3% - 9.1% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
20 μL |
灵敏度 |
2.57 pg/mL |
检测范围 |
31.25 pg/mL - 2000 pg/mL |
回收率 |
92% - 104% |
平均回收率 |
0.97 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠IL-6抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IL-6与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-6的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
IL6 分子靶点信息概述
- 分子名:IL6, interleukin 6
- 基因家族:Interferons; Interleukins; Interleukin 6 type cytokine family
- 别名:IL-6; BSF2; HGF; HSF
- 曾用名:IFNB2
- 全称:interferon, beta 2; interleukin 6 (interferon, beta 2)
IL6 分子靶点综述
白细胞介素6(IL-6)是一种同时具备促炎性和抗炎性的细胞因子。在人类中,它由IL-6基因编码。在感染期间和受到外伤后,尤其是烧伤或其它组织损伤导致的炎症,IL-6会由T细胞和巨噬细胞分泌,促进免疫应答。研究表明,小鼠抵抗肺炎链球菌感染时需要IL-6的参与,说明IL-6在抵抗感染中发挥作用。IL-6在很多疾病如糖尿病、动脉粥样硬化、抑郁症、阿尔茨海默氏病、系统性红斑狼疮、多发性骨髓瘤、前列腺癌、白塞氏病和类风湿性关节炎等中可促进炎性和自身免疫疾病进程。晚期/转移性癌症患者的血液中具有较高水平的IL-6。
大鼠 Rat Il6 分子靶点信息
- 分子名:Il6, interleukin 6
- 别称:
- Ifnb2
- IL-6
- ILg6
- Interleukin 6 (interferon beta 2)
- Interleukin 6 (interferon, beta 2)
- interleukin-6
- 基因序列:NCBI_Gene: 24498
- 蛋白序列:UniProtKB: P20607
大鼠 Rat Il6 靶点分子功能(预测)
Enables cytokine activity and interleukin-6 receptor binding activity. Involved in several processes, including cellular response to lipid; positive regulation of cell communication; and response to peptide hormone. Located in cytoplasm and extracellular space. Used to study several diseases, including acute necrotizing pancreatitis; disseminated intravascular coagulation; fatty liver disease; hypertension (multiple); and impotence. Biomarker of several diseases, including acute necrotizing pancreatitis; artery disease (multiple); auditory system disease (multiple); iron deficiency anemia; and lung disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and periodontal disease (multiple). Orthologous to human IL6 (interleukin 6).
操作步骤
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引用文献
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- 细胞培养上清
Hyaluronic Acid Coated Bilirubin Nanoparticles Attenuate Ischemia Reperfusion-Induced Acute Kidney Injury 
- 血清
Α-Mangostin Alleviated Inflammation in Rats with Adjuvant-Induced Arthritis by Disrupting Adipocytes-Mediated Metabolism-Immune Feedback 
- 血清
Jintiange Capsule Alleviates Rheumatoid Arthritis and Reverses Changes of Serum Metabolic Profile in Collagen-Induced Arthritic Rats 
- 全血 心组织 组织匀浆 细胞培养上清
Red Blood Cell Membrane-Camouflaged PLGA Nanoparticles Loaded With Basic Fibroblast Growth Factor for Attenuating Sepsis-Induced Cardiac Injury 
- 组织匀浆 结肠
Nanoparticle-assembled bioadhesive coacervate coating with prolonged gastrointestinal retention for inflammatory bowel disease therapy 
- 组织匀浆 结肠
Protective role of ergothioneine isolated from Pleurotus ostreatus against dextran sulfate sodium-induced ulcerative colitis in rat model 
Dexmedetomidine Preconditioning May Attenuate Myocardial Ischemia/Reperfusion Injury by Down-Regulating the Hmgb1-Tlr4-Myd88-Nf-Кb Signaling Pathway 
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